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Simple technique of codenaturation and fluorescence in situ hybridization with probes in a microcamera
Bruno Bossi1, Alberto Comino, Margherita Dalmasso
1Laboratorio Citogenetica, Unità Operativa Anatomia Patologica, Via Michele Coppino 26, 12100, Cuneo, Italy. anapat@scroce.sanitacn.it
Cancer Genetics and Cytogenetics
|January 29, 2003
Abstract:
We describe a rapid, precise and economical method of performing fluorescence in situ hybridization with probes in a microcamera, without chemical risk for the operator caused by the use of formamide. The application has been tried on metaphase spreads and interphase nuclei from peripheral blood or bone marrow cultures and on formalin-fixed, paraffin-embedded tissue.