Related Experiment Videos
UV induces VEGF through a TNF-alpha independent pathway
Maria G Kosmadaki1, Mina Yaar, Bennett L Arble
1Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts 02118, USA. mkosmada@bu.edu
Summary
Blocking tumor necrosis factor-alpha (TNF-alpha) did not inhibit UV-induced vascular endothelial growth factor (VEGF) in keratinocytes. UV-induced VEGF expression involves multiple pathways, not solely TNF-alpha signaling.
Area of Science:
- Dermatology
- Molecular Biology
- Cell Biology
Background:
- Vascular endothelial growth factor (VEGF) promotes angiogenesis and is produced by keratinocytes.
- Tumor necrosis factor-alpha (TNF-alpha) is implicated in UV-induced VEGF production in keratinocytes.
Purpose of the Study:
- To investigate if blocking TNF-alpha receptors inhibits UV-induced VEGF expression and secretion in keratinocyte cell lines.
- To elucidate the role of TNF-alpha in the UV irradiation response pathway of keratinocytes.
Main Methods:
- UV irradiation of SCC-12F keratinocyte cell line.
- Treatment with a TNF-alpha receptor blocking hexapeptide (Ac-KWIIVW-NH2).
- Quantification of VEGF mRNA induction and secretion via conditioned medium analysis.
Main Results:
- UV irradiation significantly induced VEGF mRNA and secretion in SCC-12F cells.
- TNF-alpha dose-dependently induced VEGF expression and secretion.
- The TNF-alpha blocking peptide inhibited TNF-alpha-induced, but not UV-induced, VEGF mRNA expression.
- Blocking TNF-alpha signaling post-UV irradiation did not affect VEGF mRNA induction or secretion.
Conclusions:
- UV-induced VEGF expression in keratinocytes is mediated by multiple signaling pathways.
- Targeting only the TNF-alpha pathway is insufficient to block UV-induced VEGF production.
- Further research is needed to identify and target alternative pathways involved in UV-induced angiogenesis.