Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural evidence for substrate strain in antibody catalysis.

Jun Yin1, Scott E Andryski, Albert E Beuscher

  • 1Department of Chemistry, University of California, Berkeley, CA 94720, USA.

Proceedings of the National Academy of Sciences of the United States of America
|January 29, 2003
PubMed
Summary

Antibody 7G12 catalyzes reactions by distorting its substrate, mesoporphyrin, towards a transition state. This study reveals how immune system evolution enhances antibody catalytic activity through structural changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lead iodide perovskite light-emitting field-effect transistor.

Nature communications·2015
Same author

Polypeptide-b-Poly(Phenyl Isocyanide) Hybrid Rod-Rod Copolymers: One-Pot Synthesis, Self-Assembly, and Cell Imaging.

Macromolecular rapid communications·2015
Same author

Multi-stimuli-responsive chiral organogels based on peptide derivatives.

Soft matter·2015
Same author

Construction of photoswitchable rotaxanes and catenanes containing dithienylethene fragments.

Organic & biomolecular chemistry·2015
Same author

Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.

Methods in molecular biology (Clifton, N.J.)·2015
Same author

Effectiveness of laparoscopic Roux-en-Y gastric bypass on obese class I type 2 diabetes mellitus patients.

Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery·2015

Area of Science:

  • Biochemistry
  • Structural Biology
  • Immunology

Background:

  • Ferrochelatase antibodies are crucial in understanding enzyme catalysis.
  • Antibody 7G12 targets mesoporphyrin, a substrate involved in porphyrin metabolism.

Purpose of the Study:

  • To elucidate the structural basis of catalytic activity in ferrochelatase antibody 7G12.
  • To understand the molecular mechanism of antibody-mediated porphyrin metallation.
  • To investigate the role of antibody evolution in enhancing catalytic efficiency.

Main Methods:

  • X-ray crystallography was used to determine the structures of antibody-bound complexes.
  • Structures were solved to 2.6-A resolution for the Michaelis complex and germ-line precursor complex.
  • Comparative analysis of germ-line and affinity-matured antibody structures.

Related Experiment Videos

Main Results:

  • The antibody-bound mesoporphyrin adopts a nonplanar conformation, indicating substrate distortion.
  • Catalysis occurs by straining the substrate towards the transition-state configuration.
  • Structural differences between germ-line and affinity-matured antibodies explain evolved binding energy and catalysis.

Conclusions:

  • Antibody 7G12 employs substrate strain to achieve catalytic activity in porphyrin metallation.
  • The evolution of antibody 7G12 demonstrates a mechanism for enhancing catalytic function through structural adaptation.
  • Understanding these mechanisms provides insights into antibody engineering and enzyme catalysis.