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Related Experiment Videos

Slug expression during organogenesis in mice.

Kathleen F Oram1, Ethan A Carver, Thomas Gridley

  • 1Jackson Laboratory, Bar Harbor, Maine 04609, USA.

The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology
|January 29, 2003
PubMed
Summary

The mouse Slug gene, a transcriptional repressor, is highly expressed in developing organs, craniofacial mesenchyme, bone, and heart structures during organogenesis.

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Area of Science:

  • Developmental Biology
  • Molecular Genetics

Background:

  • The Slug gene encodes a zinc finger-containing transcriptional repressor crucial for development.
  • Understanding Slug gene expression patterns is vital for comprehending organogenesis.

Purpose of the Study:

  • To investigate the expression patterns of the mouse Slug gene during embryonic development.
  • To identify specific tissues and cell types where Slug is actively expressed.

Main Methods:

  • Utilized a targeted Slug(lacZ) knock-in mouse model.
  • Employed histochemical detection of beta-galactosidase activity to visualize Slug expression.
  • Examined expression during organogenesis and late fetal development.

Main Results:

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  • Slug gene expression was prominent in the mesenchymal/stromal components of various organs.
  • High Slug expression was observed in craniofacial mesenchyme.
  • Significant expression was noted in developing bone (mesodermal and neural crest origins) and heart structures (outflow tract, endocardial cushions).

    • Conclusions:

    • The mouse Slug gene plays a significant role in the development of multiple organ systems.
    • Slug expression patterns suggest its involvement in mesenchymal differentiation, bone formation, and heart development.
    • These findings provide a detailed map of Slug gene activity during key embryonic developmental stages.