In vitro uptake and metabolism of pteroylpolyglutamate by rat small intestine

Related Experiment Video

Updated: Sep 29, 2026

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
16:26

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

C H Halsted, A Reisenauer, C Back

    The Journal of Nutrition
    |April 1, 1976
    PubMed

    More Related Videos

    In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability10:22

    In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

    Published on: October 19, 2018

    Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine07:00

    Mechanisms Underlying Gut Hormone Secretion Using the Isolated Perfused Rat Small Intestine

    Published on: February 26, 2019

    Related Concept Videos

    Carrier-Mediated Transport01:06

    Carrier-Mediated Transport

    Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
    Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
    Methods for Studying Drug Absorption: In vitro01:16

    Methods for Studying Drug Absorption: In vitro

    In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
    The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
    Methods for Studying Drug Absorption: In situ01:09

    Methods for Studying Drug Absorption: In situ

    In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
    The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...