Related Experiment Video
Updated: Aug 11, 2026
10:21
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
[beta-Cyclodextrin bonded stationary phases for chiral separation in HPLC]
1Institute of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China.
Se Pu = Chinese Journal of Chromatography
|January 30, 2003
Summary
This review details the design and synthesis of beta-cyclodextrin bonded stationary phases for chiral liquid chromatography. It highlights advancements in creating these crucial tools for enantioselective separations.
Area of Science:
- Analytical Chemistry
- Separation Science
Context:
- Chiral separations are critical in pharmaceuticals and chemical analysis.
- Beta-cyclodextrins are versatile hosts for enantioselective recognition.
- Development of robust chiral stationary phases (CSPs) is essential for efficient chromatography.
Purpose:
- To provide a comprehensive overview of beta-cyclodextrin bonded CSPs.
- To discuss the design strategies and synthetic methodologies for these phases.
- To consolidate current knowledge and identify future research directions.
Summary:
- This paper reviews beta-cyclodextrin bonded stationary phases used in chiral liquid chromatography.
- It focuses on the design principles and synthesis techniques employed in their development.
- The review covers 53 relevant references, offering a detailed perspective on the field.
Impact:
- Informs researchers on the latest developments in beta-cyclodextrin CSPs.
- Aids in the selection and development of appropriate chiral separation methods.
- Contributes to the advancement of enantioselective chromatography techniques.
Related Concept Videos
01:10Chromatography: Introduction
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
01:17Gas Chromatography: Types of Columns and Stationary Phases
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
01:11High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
01:05High-Performance Liquid Chromatography: Elution Process
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
01:09Ion-Exchange Chromatography
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
01:08Size-Exclusion Chromatography
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Silica particles offer advantages such as rigidity,...