[Determination of bentazon and 2,4-D butyl in mixture formulation by HPLC]

Bentazon and 2,4-D butyl(the active ingredients of Cao Di) were determined by reversed phase HPLC aimultancously using methanol/acetonitrile/citrate buffer(pH 3.1) as a mobile phase on Shim-pack clc-CN column. Results showed that the linear correlation and recovery of 2,4-D butyl were 1.000 and (100.2 +/- 0.8)% respectively, and those of bentazon were 1.000 and (98.6 +/- 0.9)% respectively. The coefficients of variation for bentazon and 2,4-D butyl were all was 0.37%.