[Determination of beta-carotene in tobacco by HPTLC]

This paper reports the high performance thin-layer chromatography (HPTLC) determination of beta-carotene in tobacco. The developer used was V(benzene):V(acetone) = 4:1. The detection wavelength was 450 nm. The linear range and the detectable limit of the method were 50 ng-0.5 microgram and 20 ng per spot of beta-carotene respectively. The method has been proved to be simple, sensitive, accurate and reproducible.