Related Experiment Video
Updated: Sep 27, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Enhanced expression of the UROC28 gene in human breast cancer: relationship to ERBB2 gene expression
Chinatsu Kamagata1, Naoki Tsuji, Kei Kondoh
1Department of Laboratory Diagnosis and Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan.
Background:
ERBB2, a highly important oncogene in invasive breast cancer, is not only a prognostic factor but also a predictive marker for response to therapeutic agents. Recently, An et al. (10) identified a novel gene, UROC28, that is also overexpressed in breast cancer. To examine possible interrelationships, we quantitated UROC28 and ERBB2 mRNA in breast cancers.
Materials And Methods:
The expression of UROC28 and ERBB2 mRNA in breast cancer tissues were determined using RT-PCR. The expression was also examined in T-47D breast cancer cells treated with estrogen.
Results:
UROC28 mRNA expression was greater in cancers than in noncancerous tissues (p < 0.0001), as was ERBB2 mRNA. ERBB2 and UROC28 gene expression was dose-dependently down-regulated in T-47D breast cancer cells treated with estradiol. However UROC28 mRNA and ERBB2 mRNA expression did not correlate with one another.
Conclusion:
These results indicate that UROC28 may be a useful target molecule in breast cancer diagnosis and treatment, complementing ERBB2.
Related Concept Videos
02:38Mitogens and the Cell Cycle
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...