Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microfluidic chip-based method for genotyping microsatellites, VNTRs and insertion/deletion polymorphisms.

Youvraj R Sohni1, James P Burke, Peter J Dyck

  • 1Translational Genomics Center, Mayo Clinic, Rochester, MN 55905, USA.

Clinical Biochemistry
|January 30, 2003
PubMed
Summary

A novel microfluidic chip system rapidly genotypes variable number of tandem repeats (VNTRs) and insertion/deletion polymorphisms. This method accurately analyzes genetic variations linked to diseases like diabetes and hypertension.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Distinctive Pattern of Pathologic Abnormality in Amyloid-like Monoclonal IgM Deposition Neuropathy.

Mayo Clinic proceedings·2025
Same author

Distinctive clinical features in biopsy-proven nerve large-arteriole vasculitis and microvasculitis.

Brain : a journal of neurology·2024
Same author

Neurologic Clinical, Electrophysiologic, and Pathologic Characteristics of Primary vs Secondary Neurolymphomatosis.

Neurology·2024
Same author

Nerve transcriptomes in autoimmune and genetic demyelinating neuropathies: Pathogenic pathway assessment of nerve demyelination.

Journal of neuroimmunology·2023
Same author

Descriptive, real-world treatment patterns, resource use, and total cost of care among eculizumab- and ravulizumab-treated members with paroxysmal nocturnal hemoglobinuria.

Journal of managed care & specialty pharmacy·2023
Same author

Sural nerve biopsy utility by masked assessment of individual histologic preparations.

Journal of the peripheral nervous system : JPNS·2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Variable number of tandem repeats (VNTRs) and insertion/deletion polymorphisms are crucial genetic markers.
  • Association of specific VNTRs and polymorphisms in nitric oxide synthase (iNOS5, eNOS27) and angiotensin-converting enzyme (ACE/ID) genes with various diseases is established.
  • Efficient and accurate genotyping methods are needed for large-scale genetic studies.

Purpose of the Study:

  • To develop and validate an integrated microfluidic chip-based system for genotyping VNTRs and insertion/deletion polymorphisms.
  • To apply this system for analyzing specific genetic loci (iNOS5, eNOS27, ACE/ID) associated with diabetic complications, hypertension, and cardiovascular diseases.

Main Methods:

  • Development of a microfluidic chip-based system for DNA fragment analysis.

Related Experiment Videos

  • Genotyping of three specific genetic loci: iNOS5 pentanucleotide repeat, eNOS27 VNTR, and ACE/ID insertion/deletion polymorphism.
  • Validation of results using bi-directional sequencing and GeneScan analysis.

    • Main Results:

    • The microfluidic system successfully genotyped 230 DNA samples for iNOS5, eNOS27, and ACE/ID polymorphisms.
    • Genotyping results showed clear separation and accurate sizing of DNA fragments, with 100% concordance upon validation.
    • The system demonstrated efficiency in both DNA fragment separation, sizing, and quantitation.

    Conclusions:

    • The integrated microfluidic chip-based system provides a rapid, accurate, and efficient method for genotyping VNTRs and insertion/deletion polymorphisms.
    • This technology is suitable for both small- and large-scale genotyping studies.
    • The developed method can be applied to analyze genetic variations associated with various complex diseases.