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[Establishment of transformation system of Streptomyces lincolnensis]
1Department of Biochemical Engineering, East China University of Science and Technology, Shanghai 200237.
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 31, 2003
Abstract:
Transformation system of S. lincolnensis was established to improve lincomycin--producing strains by Gene retransformation Technology. Under optimal conditions, 10(11) protoplasts were prepared from 25 ml SM culture medium, and the regeneration frequency of protoplasts was 16%. The transformation frequency of pIJ702 was up to 5.5 x 10(5)/microgram DNA. The results revealed that gene manipulation in S. lincolnensis was facile as that in S. lividans.