[Detection of hypodermal and hematopoietic necrosis baculovirus by PCR method]

For control of the explosive epidemic disease of Penaeid shrimp in China. We have developed a method for detection of Hypodermal and hematopoietic necrosis baculovirus (HHNBV) by polymerase chain reaction (PCR). Two pairs of PCR primers (DP1/DP2 and DP3/DP4) were prepared, based on the RFLP analysis between HHNBV and penaeid rod-shaped DNA virus. PCR using either DP1/DP2 or DP3/DP4 specified HHNBV genome. When PCR products amplified with DP1/DP2 were subjected to nested PCR with DP3/DP4, a high sensitivity about 0.04 pg was seem with the nested PCR.