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Updated: Sep 27, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[A study on taxonomy of rhizobia isolated from Kummerwia sp. and Indigofera sp]
1Dept Resource and Environment, Northwest Agricultural University, Yangling 712100.
Abstract:
The rhizobial strains isolated from Kummerowia and Indigofera and the known reference strains were classified by performing numerical taxonomy. New isolated strains were divided into two new clusters at 83% similarity level. Based on the numerical taxonomy, additional isolates in each cluster were studied by using SDS-PAGE of whole-cell protein. Twenty-four strains isolated from Kummerowia fell into cluster 1. Twenty strains isolated from Indigofera fell into cluster 2. The results of G + C mol% and DNA homology analysis showed that the DNA homologies between the central strain SH713 and SHL042 and the 13 type strains were less than 61%. Thus, the rhizobial strains from Kummerowia and Indigofera were two new individual species of Rhizobium.
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