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[Sugar phosphorylation activities in acetogenic bacteria].

W Jiang1, J A Patterson

  • 1Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|January 31, 2003
PubMed
Summary

Seven acetogenic bacteria were analyzed for glucose phosphorylation. Significant variations in PEP- and ATP-dependent phosphorylation rates were observed, with some bacteria likely utilizing a glucose phosphotransferase system (PTS).

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Area of Science:

  • Microbiology
  • Biochemistry

Context:

  • Acetogenic bacteria play crucial roles in anaerobic environments.
  • Understanding their carbohydrate metabolism is key to their ecological function.

Purpose:

  • To investigate the PEP- and ATP-dependent phosphorylation of glucose and 2-deoxyglucose in seven acetogenic bacterial species.
  • To identify the presence and characteristics of glucose phosphotransferase systems (PTS) in these bacteria.

Summary:

  • All seven tested acetogenic bacteria exhibited glucose phosphorylation activity, but rates varied significantly.
  • Isolate A10, H3HH, and E. limosum likely possess a glucose PTS, while A. ruminis, A. woodii, and isolates A2, A4 showed minimal PEP-dependent activity.
  • ATP-dependent phosphorylation rates were generally higher than PEP-dependent rates across all species. Glucose PTS in isolates A10 and H3HH were induced by extracellular glucose and increased with culture growth phase.

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  • Maltose and sucrose phosphorylation were also detected in isolates A10 and H3HH, with activity rates varying by sugar and organism.

    • Impact:

    • This study reveals diverse glucose uptake mechanisms among acetogenic bacteria.
    • Findings contribute to understanding microbial energy metabolism and carbohydrate utilization in anaerobic ecosystems.