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Updated: Sep 27, 2026
Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
Published on: May 31, 2022
[Influence of peritoneal dialysate on nitric oxide production and viability of macrophage]
Ze-Ran Yang1, Ji-Cheng Li, Kai Zhang
1College of Medical Sciences, Zhejiang University,Hangzhou 310031,China.
Abstract:
OBJECTIVE: To investigate the influence of commercial peritoneal dialysate (CDS) on function of macrophage. METHODS: Cultured peritoneal macrophages were divided into two experimental groups and their controls.(1) Macrophages were cultured in conditioned culture medium containing 50%CDS (0.139 mol/L glucose) for 24 h. (2)Macrophage were exposed to CDS containing 0.139mol/L glucose for 10, 30 and 60 min respectively, and then cultured in CDS-free medium for 24 h. The nitric oxide (NO) production and MTT in two groups were measured. In each control group, CDS was replaced by same amount of culture medium. RESULTS: NO production and MTT reduction ability (related to cell viability) of experimental groups were remarkably lower than those of controls and the NO production and MTT reduction in 60min CDS-exposed group were lower than that of 10 min and 30 min CDS-exposed group. CONCLUSION: Dialysate may have detrimental effects on viability and other function of macrophage and these effects may related to time length of CDS exposure.
Insights
Commercial peritoneal dialysate (CDS) may harm macrophage function and viability. Longer exposure times to CDS result in greater reductions in nitric oxide production and cell viability, indicating potential detrimental effects.
Area of Science:
- Peritoneal dialysis research
- Immunology
- Cell biology
Background:
- Macrophages play a crucial role in the peritoneal immune response.
- Peritoneal dialysis solutions (dialysates) are used to remove waste products from the body.
- The impact of commercial dialysates on macrophage function requires further investigation.
Purpose of the Study:
- To evaluate the effects of commercial peritoneal dialysate (CDS) on macrophage function.
- To assess the influence of CDS exposure duration on macrophage nitric oxide (NO) production and cell viability.
Main Methods:
- Cultured peritoneal macrophages were exposed to 50% CDS (0.139 mol/L glucose) for 24 hours.
- Macrophages were exposed to CDS for 10, 30, and 60 minutes, followed by culture in CDS-free medium.
- Nitric oxide (NO) production and MTT assay (for cell viability) were measured in experimental and control groups.
Main Results:
- Macrophages exposed to CDS exhibited significantly lower NO production and MTT reduction compared to controls.
- A dose-dependent relationship was observed, with longer CDS exposure (60 min) leading to greater reductions in NO production and cell viability.
- These findings suggest that CDS may impair macrophage function and viability.
Conclusions:
- Commercial peritoneal dialysate may have detrimental effects on macrophage viability and function.
- The duration of CDS exposure appears to be a critical factor influencing these detrimental effects.
- Further research is warranted to understand the mechanisms behind dialysate-induced macrophage dysfunction.
