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Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model
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[Analysis of virulence-related proteins of Streptococcus suis type 2 from swine Streptococcus isolated in China]

Yu Ou1, Chengping Lu

  • 1Key Lab Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 1, 2003
PubMed

Insights

This study investigated Streptococcus suis type 2 virulence proteins, muraminidase-released protein (MRP) and extracellular factor (EF), in various isolates. Most strains lacked both key virulence factors, suggesting potential for attenuated vaccine development.

Area of Science:

  • Microbiology and Immunology
  • Bacterial Pathogenesis
  • Veterinary Infectious Diseases

Context:

  • Streptococcus suis type 2 is a significant swine pathogen causing economic losses.
  • Virulence factors like muraminidase-released protein (MRP) and extracellular factor (EF) are crucial for S. suis pathogenicity.
  • Understanding the prevalence of these factors in different isolates is key to disease control and vaccine development.

Purpose:

  • To characterize the presence and expression of muraminidase-released protein (MRP) and extracellular factor (EF) in Streptococcus suis type 2.
  • To analyze the phenotypic variations of MRP and EF in a collection of swine, German, and human S. suis type 2 isolates.
  • To develop specific antibodies for detecting these virulence factors.

Summary:

  • Antibodies were generated against MRP and EF from S. suis type 2 isolate HA9801.
  • SDS-PAGE and immunoblotting were used to analyze 19 S. suis type 2 isolates (17 Chinese, 1 German, 1 human).
  • Results showed 11 strains produced MRP and 10 produced EF, with four observed phenotypes: MRP+ EF+ (8/19), MRP+ EF (1/19), MRP+ EF- (1/19), and MRP- EF- (10/19).

Impact:

  • Identifies a high prevalence of strains lacking both MRP and EF (MRP- EF-), suggesting potential candidates for live attenuated vaccine development.
  • Provides valuable data on the distribution of key virulence factors in S. suis type 2, aiding in epidemiological studies.
  • Contributes to a better understanding of the genetic and phenotypic diversity of S. suis virulence factors.

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