[Studies on hydantoinase producing conditions of Arthrobacter K1108]

The hydantoinase-producing conditions from strain Arthrobacter K1108 were investigated. It is shown that the hydantoinase in the strain is intracellular and inducible. Its optimal inducer is 5-benzylhydantoin, while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in K1108. A gratuitous inducer was designed, with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin. The media for culturing the bacteria were screened and optimized. Under optimal conditions, the specific activity of K1108 cells reached 10.8 U/mL.