Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[The optimization of guanosine fermentation based on process parameter correlation analysis].

Xianpeng Cai1, Shuangxi Chen, Ju Chu

  • 1State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai 2000237, China.

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 1, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic state-driven monitoring and control of abnormal pigment formation in sodium gluconate fermentation by <i>Aspergillus niger</i>.

Synthetic and systems biotechnology·2026
Same author

Electrode-Engineered Vertical MoS<sub>2</sub>/MoTe<sub>2</sub> Heterostructure for Self-Powered, Rapid, High-Sensitivity Broadband Photodetection.

ACS omega·2026
Same author

CT-based habitat imaging integrated with radiomics and clinicopathology for noninvasive prediction of microvascular invasion in hepatocellular carcinoma.

Frontiers in oncology·2026
Same author

Octadecylamine-Modified CuO NPs Enabling Highly Selective <i>In Vivo</i> Ascorbic Acid Potentiometric Detection with Enhanced Sulfide Tolerance.

ACS sensors·2026
Same author

The active ingredient Monotropein in Morinda officinalis alleviates neuroinflammation via inhibiting cGAS/STING signaling pathway.

International immunopharmacology·2026
Same author

Ultrasound-based radiomics and habitat analysis for noninvasive assessment of Ki-67 overexpression in breast cancer.

Frontiers in oncology·2026

Bacillus subtilis fermentation was optimized to boost guanosine production. Metabolic flux analysis revealed shifts that led to a significant increase in guanosine yield to 30 g/L.

Area of Science:

  • Microbial Fermentation
  • Metabolic Engineering
  • Biochemical Processes

Context:

  • Analysis of Bacillus subtilis fermentation for guanosine production.
  • Utilized on-line and off-line parameter determination.
  • Employed correlation analysis for physiological regulation and metabolic flux distribution.

Purpose:

  • To understand and optimize the fermentation process for guanosine synthesis.
  • To investigate metabolic flux shifts during guanosine production.
  • To enhance guanosine yield through process optimization.

Summary:

  • Characterized the Bacillus subtilis fermentation process for guanosine production in a 50 L fermentor.
  • Identified a metabolic flux shift from the HMP pathway to the EMP and TCA cycles.

Related Experiment Videos

  • Preliminary analysis of flux shift reasons informed process optimization, increasing guanosine yield to 30 g/L.

    • Impact:

    • Successfully increased guanosine yield to 30 g/L.
    • Provides insights into metabolic regulation for enhanced bioproduction.
    • Optimized fermentation strategy for industrial-scale guanosine synthesis.