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[Sequencing and analysis of flocculation gene(FLO1G)]
Xiuping He1, Wenjie Guo, Borun Zhang
1Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 1, 2003
Summary
The flocculation gene (FLO1G) was sequenced, revealing a protein-coding region. Despite a deletion, the gene retains strong flocculation ability, indicating the deleted part is not essential.
Area of Science:
- Molecular Biology
- Yeast Genetics
Background:
- Flocculation is a crucial trait in yeast, influencing brewing and industrial processes.
- The FLO1 gene is known to confer strong flocculation in Saccharomyces cerevisiae.
Purpose of the Study:
- To determine the sequence of the flocculation gene (FLO1G).
- To analyze the impact of a specific deletion on FLO1G's flocculation function.
Main Methods:
- DNA sequencing of the FLO1G gene.
- Homologous analysis comparing FLO1G to FLO1.
- Functional analysis by expressing FLO1G in a non-flocculent yeast strain.
Main Results:
- The FLO1G open reading frame (ORF) is 3936 bp, encoding a 1312 amino acid protein.
- FLO1G is homologous to FLO1 but contains a 675 bp deletion within an ORF repeat region.
- Expression of the cloned FLO1G fragment induced strong flocculence in the non-flocculent strain S. cerevisiae YS58.
Conclusions:
- The deletion in FLO1G does not abolish its flocculation capability.
- The removed sequence is not critical for the gene's function in promoting yeast flocculence.