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Indomethacin-induced morphologic changes in the rat urinary bladder epithelium.
Sule Cetinel1, B lent Cetinel, Feriha Ercan
1Department of Histology and Embryology, Marmara University School of Medicine, Haydarpaşa, Istanbul, Turkey.
Urology
|February 1, 2003
Summary
Indomethacin causes bladder damage similar to interstitial cystitis in rats, including leaky epithelium and increased mast cells. Further human studies are needed to confirm the incidence of nonsteroidal anti-inflammatory drug-induced cystitis.
Area of Science:
- Urology
- Pharmacology
- Histopathology
Background:
- Nonsteroidal anti-inflammatory drug (NSAID)-induced cystitis is an under-recognized condition.
- Indomethacin, like tiaprofenic acid, has been linked to NSAID-induced cystitis.
Purpose of the Study:
- To investigate the morphologic changes in rat urothelium caused by indomethacin.
- To characterize the histopathological effects of indomethacin on the bladder.
Main Methods:
- Rats were divided into control, high-dose indomethacin (20 mg/kg IP), and therapeutic-dose indomethacin (3.25 mg/kg/day oral for 3 weeks) groups.
- Bladders were examined using light and electron microscopy.
Main Results:
- Indomethacin induced focal epithelial degeneration, more severe in the high-dose group.
- Both indomethacin groups showed increased mucosal mast cells and lanthanum nitrate penetration, indicating increased epithelial permeability (P <0.0001).
- Mast cell counts differed significantly between high-dose and therapeutic-dose groups (P <0.0001).
Conclusions:
- Indomethacin administration resulted in histopathologic changes consistent with interstitial cystitis, including urothelial "leakiness" and mucosal mastocytosis.
- Prospective clinical trials are necessary to determine the actual incidence of NSAID-induced cystitis in humans.