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Erythrocyte sedimentation rate and C-reactive protein
Klinische Wochenschrift
|February 15, 1976
Summary
High levels of C-reactive protein (CRP) from cancer patients slightly increased erythrocyte sedimentation rate in healthy plasma. A new method allows simultaneous measurement of CRP and other acute phase reactants.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- C-reactive protein (CRP) is an acute phase reactant.
- Elevated CRP levels are associated with inflammation and certain diseases.
- The direct impact of isolated CRP on erythrocyte sedimentation rate (ESR) requires further investigation.
Purpose of the Study:
- To investigate the effect of isolated C-reactive protein (CRP) on erythrocyte sedimentation rate (ESR).
- To develop a method for simultaneous quantitative determination of CRP and other acute phase reactants.
Main Methods:
- Isolation of CRP from pleural exudate of cancer patients.
- Addition of isolated CRP to plasma from healthy blood donors.
- Measurement of ESR following CRP addition.
- Development of an immunoelectrophoretic method for simultaneous quantification of CRP and C3 or other acute phase reactants.
Main Results:
- Significant increase in ESR was observed only with excessive CRP concentrations (220 mg/ml).
- A novel immunoelectrophoretic technique was successfully developed for simultaneous determination of CRP and other acute phase proteins.
Conclusions:
- Isolated CRP has a limited effect on ESR unless present in very high concentrations.
- The developed immunoelectrophoretic method offers a valuable tool for simultaneous analysis of multiple acute phase reactants in clinical diagnostics.