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Structural characterization of the O-chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a
Suzon Larocque1, Jean-Robert Brisson, Hélène Thérisod
1Institute for Biological Sciences, NRC, Ottawa, ON, Canada.
FEBS Letters
|February 1, 2003
Abstract:
The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI studies showed the O-chain to be a polymer composed of 1,4-linked 2-acetamidino-3-[3-hydroxybutanamido]-2,3-dideoxy-beta-D-glucopyranosyluronic acid residues.