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Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
Published on: November 16, 2012
[Influence of pH control on the production of curdlan by Alcaligenes faecalis strain]
Lei Wang1, Xiao-Bei Zhan, Yi-Hui Zhu
1Key Laboratory of Industrial Biotechnology of Ministry of Education, Southern Yangtze University, Wuxi 214036, China.
Abstract:
A two-stage pH control method was employed in batch fermentation of curdlan by Alcaligenes faecalis WX-C12 where cell-growth stage was constantly controlled at pH 7.0 and stationary stage was controlled at a constant pH as well. The influence of pH control on the curdlan production was investigated. The optimal pH control of batch process for curdlan production was obtained when cell-growth stage was controlled at pH 7.0 and stationary stage was constantly controlled at pH 5.6. Production and productivity of curdlan, QP and YP/S reached 28.19 g/L, 291 mg/(L.h), 132.27 mg/(L.h.g) and 0.659, an improvement of 20.4%, 38.1%, 38.1% and 29.5% compared to a pH uncontrolled operation respectively.
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