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[Identification of down-regulated expressed sequence tag at chromosome 3p21 in nasopharyngeal carcinoma]
Xiu-Sheng He1, Zhu-Chu Chen, Fang Tian
1Cancer Research Institute, Central South University, Changsha, Hunan, 410078, P. R. China.
Background & Objective:
It was reported that a locus of high frequency loss of heterozygosity was found at 3p14-25 of nasopharyngeal carcinoma (NPC) cells. This study was designed to identify the expressed sequence tags (ESTs) associated with NPC at chromosome 3p21 in order to clone new candidate NPC-associated genes at the locus.
Methods:
The expression of relative ESTs at 3p21 was determined in nasopharyngeal carcinoma tissues and normal nasopharyngeal epithelia using ESTs homology analysis with bioinformatics in computer network combined with reverse transcription-polymerase chain reaction (RT-PCR). The expression of EST was detected in other normal tissues and tumor cell lines using Northern blot analysis.
Results:
Comparing with expression of normal nasopharyngeal epithelia, EST (N31985) was down-regulated in 60.00% (3/5) nasopharyngeal carcinoma cell lines and 47.06% (16/34) nasopharyngeal carcinoma tissues (P < 0.05).
Conclusion:
N31985 is down-regulated in NPC, suggesting it may play a role in NPC carcinogenesis.
