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Heat modifiability of outer membrane protein of Pasteurella multocida serotype B:2
Anirban Pal1, S K Srivastava, V P Singh
1Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar 243 122, India. drapaul@yahoo.com
Abstract:
Outer membrane proteins (OMP) are generally porins, functioning as molecular sieves assisting in the transmembrane transportation. Heat modifiable characteristics of OMP from P. multocida B: 2 have been explored to know their basic characteristics on event of temperature rise. A major band of 32 kDa and two minor bands of approximately 39 and approximately 28 kDa were found to be heat modifiable. It is suggested that boiling at 100 degrees C in presence of beta mercaptoethanol for 5 min is sufficient for characterisation of OMP by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis.
Insights
Outer membrane proteins (OMP) from P. multocida B:2 exhibit heat-modifiable characteristics. Boiling at 100°C for 5 minutes is sufficient for their characterization using SDS-PAGE.
Area of Science:
- Microbiology
- Protein Biochemistry
Background:
- Outer membrane proteins (OMPs) are crucial for bacterial transmembrane transport, often functioning as porins.
- Understanding the characteristics of OMPs from specific pathogens like P. multocida B:2 is essential for further research.
Purpose of the Study:
- To investigate the heat-modifiable characteristics of Outer membrane proteins (OMPs) from P. multocida B:2.
- To determine the optimal conditions for OMP characterization.
Main Methods:
- Analysis of Outer membrane proteins (OMPs) from P. multocida B:2.
- Application of heat treatment (boiling at 100°C for 5 minutes) in the presence of beta-mercaptoethanol.
- Characterization using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Main Results:
- Identified a major heat-modifiable OMP band at 32 kDa.
- Detected two minor heat-modifiable OMP bands at approximately 39 kDa and 28 kDa.
- Demonstrated that boiling at 100°C for 5 minutes is adequate for OMP characterization.
Conclusions:
- The Outer membrane proteins (OMPs) of P. multocida B:2 display heat-modifiable properties.
- Standard heat treatment conditions are effective for the electrophoretic characterization of these OMPs.