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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
Published on: October 18, 2019
Cloning and sequencing of beta toxin gene of Clostridium perfringens type C
P P Goswami1, K S Girish, P Chaudhuri
1National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar 243 122, India.
Indian Journal of Experimental Biology
|February 4, 2003
Abstract:
A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.
