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Mouse Models Of Helicobacter Infection And Gastric Pathologies
07:43

Mouse Models Of Helicobacter Infection And Gastric Pathologies

Published on: October 18, 2018

Acute inflammatory response in the stomach of BALB/c mice challenged with coccoidal Helicobacter pylori

E M A Rabelo-Gonçalves1, N F Nishimura, J M R Zeitune

  • 1Laboratório de Bacteriologia, Centro de Diagnóstico de Doenças do Aparelho Digestivo-Gastrocentro, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brasil. emargoncalves@yanoo.com.br

Memorias Do Instituto Oswaldo Cruz
|February 4, 2003
PubMed

Insights

Coccoid Helicobacter pylori can cause inflammation in mouse stomachs, especially after reinfection. This study confirms its pathogenicity and inflammatory potential in a murine model.

Area of Science:

  • Microbiology
  • Pathology
  • Immunology

Background:

  • Helicobacter pylori is a significant human pathogen.
  • The coccoid form of H. pylori is often considered non-viable, but its role in infection requires further investigation.

Purpose of the Study:

  • To evaluate the viability and pathogenicity of coccoid Helicobacter pylori in a murine model.
  • To investigate the inflammatory response induced by coccoid H. pylori infection.

Main Methods:

  • BALB/c mice were inoculated intragastrically with coccoid H. pylori.
  • Mice were divided into single-infection and two-day reinfection groups, with a control group.
  • Gastric and duodenal tissues were collected at various time points post-inoculation for histopathological examination using hematoxylin-eosin and Giemsa staining.

Main Results:

  • Reinfected mice exhibited mild-to-moderate gastric inflammatory infiltrate by day 14, predominantly lymphomonocytic.
  • Severe eosinophilic infiltration was observed in the gastric corpus by day 21 in reinfected mice.
  • Single-infected mice showed lymphomonocytic infiltration from day 14 onwards.

Conclusions:

  • Coccoid H. pylori is viable and pathogenic in a murine model.
  • Coccoid H. pylori can induce an acute inflammatory response in the stomach, particularly in reinfected animals.
  • The study highlights the importance of considering the coccoid form of H. pylori in infection pathogenesis.

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