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Updated: Sep 27, 2026
Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice
Published on: July 2, 2018
[Is blood squeezed out from the microcirculation soon after coronary occlusion?: real time myocardial contrast
Kentaro Otani1, Shintaro Beppu, Kaori Ibaraki
1School of Allied Health Sciences, Osaka University, Faculty of Medicine, Osaka, Yamadaoka 1-7, Suita, Osaka 565-0871.
Insights
Blood remains in myocardial microvessels after coronary artery occlusion, not being squeezed out by residual contractions. This study used real-time contrast echocardiography to examine microcirculation changes following coronary occlusion in dogs.
Area of Science:
- Cardiovascular Research
- Myocardial Microcirculation
- Diagnostic Imaging
Background:
- The fate of blood within myocardial microvessels post-coronary artery occlusion remains unclear.
- Residual myocardial contraction might potentially expel trapped blood.
- Real-time contrast echocardiography was employed to investigate microcirculation dynamics.
Purpose of the Study:
- To elucidate the fate of blood in myocardial microvessels after coronary artery occlusion.
- To assess whether residual contractions expel blood from the microvasculature.
- To examine microcirculatory changes using real-time contrast echocardiography.
Main Methods:
- 17 open-chest dogs underwent left circumflex coronary artery ligation for 15 seconds.
- Myocardial opacification was assessed using FS69 (Optison) contrast agent and Coherent Contrast Imaging.
- Video intensity of the risk area was measured, and wall motion abnormalities (normokinesis to akinesis) were evaluated.
Main Results:
- Coronary occlusion significantly decreased video intensity in the risk area.
- Wall motion deteriorated progressively, with akinesis developing at 7.8 ± 0.8 seconds post-ligation.
- Changes in video intensity during wall motion deterioration were minimal and not statistically significant.
Conclusions:
- Blood persists within the myocardial vascular bed immediately after coronary occlusion.
- Residual myocardial contractions do not appear to effectively expel blood from the microvasculature.
- Findings suggest blood stasis rather than expulsion occurs post-occlusion.
Background And Objectives:
The fate of blood trapped inside the myocardial microvessels after complete occlusion of the coronary artery has not been elucidated. Blood may be squeezed out by residual contraction even after coronary occlusion. The microcirculation was examined soon after coronary occlusion by real-time contrast echocardiography.
Methods:
Myocardial opacification in the short-axis view was examined during infusion of FS69 (Optison) using the Coherent Contrast Imaging method(SIEMENS Sequoia 512) in 17 open chest dogs. The left circumflex coronary artery was completely ligated for 15 sec and the video intensity (256 gray level) of the risk area was measured at end-systole of every 3 beats before and after ligation. Temporal deterioration of the wall motion was evaluated, defined as normokinesis, mild hypokinesis and severe hypokinesis, and akinesis. Changes in the video intensity during each stage of wall motion abnormalities were examined.
Results:
The video intensity of the risk area decreased significantly after coronary occlusion. Wall motion of the risk area deteriorated gradually after ligation, in which mild hypokinesis, severe hypokinesis and akinesis developed consecutively at 2.8 +/- 0.5, 5.4 +/- 0.5 and 7.8 +/- 0.8 sec after coronary ligation, respectively. The changes in video intensity during each stage of the wall motion abnormalities were small and not statistically significant, irrespective of severity of wall motion abnormalities.
Conclusions:
These findings suggest that the blood remains in the vascular bed immediately after the coronary occlusion and is not squeezed out even if wall motion of the risk area continues.
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