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Updated: Sep 27, 2026
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
Published on: August 6, 2021
Determination of D-amino acid oxidase activity in tumour cells
Taizo Sasamura1, Akihiko Matsuda, Yukifumi Kokuba
1Pharmaceutical Research Laboratories, Ajinomoto Co Inc, 1-1 Suzuki-cho, Kawasaki-ku Kawasaki, 210-8681 Japan.
Background:
We evaluated the assay for determining D-amino acid oxidase (DAAO) activity in tumour cells, rat liver and rat kidney for studying the effects of D-amino acid-containing solution on cancer patients.
Methods And Results:
In this method the amount of ammonia produced by the DAAO activity after removal of endogenous ammonia using a Sephadex G25 column was determined. The highest activity was observed in rat kidney, which was almost eight times that found in rat liver. As compared with host tissues, the DAAO activity in tumour cells was considerably less.
Conclusions:
This DAAO assay may be useful for analysis of various tissue samples as well as tumour cells.
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