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Chemoenzymatic synthesis of high-mannose type HIV-1 gp120 glycopeptides
Suddham Singh1, Jiahong Ni, Lai-Xi Wang
1Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 West Lombard Street, Baltimore, MD 21201, USA.
Bioorganic & Medicinal Chemistry Letters
|February 5, 2003
Abstract:
A chemoenzymatic approach to the synthesis of glycoforms of HIV-1 gp120 glycopeptides is described. Thus, the high-mannose type glycopeptides [gp120 (336-342)] containing Man(9), Man(6) and Man(5) moieties, respectively, were synthesized in satisfactory yields via transglycosylation to the acetylglucosaminyl peptide, using the recombinant Arthrobacter Endo-beta-N-acetyl-glucosaminidase (Endo-A) as the key enzyme.