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Synthesis of labelled PNA oligomers by a post-synthetic modification approach
Beatriz G de la Torre1, Ramon Eritja
1Institut de Biologia Molecular de Barcelona, C.S.I.C., Jordi Girona 18-26, E-08034 Barcelona, Spain.
Bioorganic & Medicinal Chemistry Letters
|February 5, 2003
Abstract:
The preparation of t-butoxycarbonyl (Boc)-protected O(4)-(o-nitrophenyl) thymine peptide nucleic acid (PNA) monomer is described. This PNA monomer was incorporated into PNA oligomer sequences. The post-synthetic modification of the oligomers to yield fluorescently-labelled PNA oligomers was studied before and after the removal of the protecting groups. In both cases, the desired fluorescently-labelled PNA oligomer was obtained in good yields.