Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single-cell kinase assays: opening a window onto cell behavior.

Christopher E Sims1, Nancy L Allbritton

  • 1Department of Physiology and Biophysics, D380 Medical Sciences, University of California, Irvine, CA 92697-4560, USA.

Current Opinion in Biotechnology
|February 5, 2003
PubMed
Summary

New biochemical assays can now measure kinase activity in single mammalian cells. This breakthrough allows for simultaneous multi-kinase analysis, advancing our understanding of cell signaling and behavior.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geometrically Constrained Growth Factor Concentration Favors Enrichment of Goblet Cells and Mucus Formation.

ACS biomaterials science & engineering·2026
Same author

Development of large-scale gastruloid array to identify aberrant developmental phenotypes.

APL bioengineering·2025
Same author

Imaging 3D cell cultures with optical microscopy.

Nature methods·2025
Same author

Thin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.

Analytical chemistry·2025
Same author

Fibroblasts modulate epithelial cell behavior within the proliferative niche and differentiated cell zone within a human colonic crypt model.

Frontiers in bioengineering and biotechnology·2024
Same author

A Microphysiological System with an Anaerobic Air-Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium.

Advanced materials interfaces·2024

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Analytical techniques now enable biochemical assays at the single-cell level.
  • Kinases are crucial enzymes regulating diverse cellular processes.
  • Understanding kinase activation is key to deciphering cell physiology.

Purpose of the Study:

  • To highlight the significance of single-cell kinase assays.
  • To introduce the potential for simultaneous multi-kinase measurements.
  • To emphasize the contribution to understanding cell signaling dynamics.

Main Methods:

  • Development of advanced analytical techniques for single-cell analysis.
  • Implementation of biochemical assays to measure kinase activation.
  • Exploration of multiplexed assays for simultaneous kinase detection.

Related Experiment Videos

Main Results:

  • Biochemical assays can be successfully performed on individual mammalian cells.
  • Kinase activation can be measured at the single-cell level.
  • Simultaneous assays for multiple kinases in a single cell are becoming feasible.

Conclusions:

  • Single-cell kinase assays offer profound insights into cellular control mechanisms.
  • These assays reveal the dynamic nature of signal transduction networks.
  • Multiplexed single-cell kinase assays represent a significant advancement in cell biology research.