Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification.

Stephen F Kingsmore1, Dhavalkumar D Patel

  • 1Molecular Staging Inc., 300 George Street, New Haven, CT 06511, USA.

Current Opinion in Biotechnology
|February 5, 2003
PubMed
Summary

Multiplexed immunoassays using rolling circle amplification (RCA) protein chips offer sensitive and reproducible analysis of up to 150 proteins. These advanced protein chips show diagnostic utility and potential for identifying biomarkers for disease and drug response.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmental organization of sensory and sympathetic ganglia.

Nature·2026
Same author

Genetic Variation in Clinical Cohorts.

The New England journal of medicine·2026
Same author

Systematic review and meta-analysis of humoral immunity proteins and mortality in sepsis.

Critical care (London, England)·2025
Same author

Genome x Environment analysis of Sudden Unexpected Infant Death unveils etiologic heterogeneity and strong cannabis and genetic disease risks.

medRxiv : the preprint server for health sciences·2025
Same author

Clinical utility and cost-effectiveness of BeginNGS newborn screening by genome sequencing and standard newborn screening for severe childhood genetic diseases: an adaptive, international and comparative clinical trial.

BMJ open·2025
Same author

A phenotypic brain organoid atlas and biobank for neurodevelopmental disorders.

Cell stem cell·2025

Area of Science:

  • Biotechnology
  • Proteomics
  • Immunodiagnostics

Background:

  • Multiplexed immunoassays on protein microarrays are valuable for analyzing biological states.
  • Challenges in specificity and sensitivity are being addressed by quality control and amplification technologies.

Purpose of the Study:

  • To establish the feasibility and utility of antibody-based protein microarrays for biological analysis.
  • To highlight the role of rolling circle amplification (RCA) in enhancing protein chip performance.

Main Methods:

  • Utilizing antibody-based protein microarrays with rolling circle amplification (RCA) technology.
  • Profiling up to 150 proteins in diverse biological substrates like serum and plasma.
  • Applying multivariate statistical analysis for biomarker discovery.

Related Experiment Videos

Main Results:

  • RCA-amplified protein chips demonstrate high sensitivity, broad dynamic range, and good reproducibility.
  • Demonstrated diagnostic utility in multiplexed allergen testing.
  • Potential for identifying multiplexed biomarker classifiers when combined with statistical analysis.

Conclusions:

  • RCA-amplified protein chips are a powerful tool for high-throughput proteomic analysis.
  • These chips have significant potential for disease diagnosis and predicting drug response.
  • The technology overcomes previous limitations in sensitivity and specificity for protein profiling.