Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DPI induces mitochondrial superoxide-mediated apoptosis.

Nianyu Li1, Kathy Ragheb, Gretchen Lawler

  • 1Purdue University, Department of Basic Medical Sciences, West Lafayette, IN 47907, USA.

Free Radical Biology & Medicine
|February 5, 2003
PubMed
Summary

Diphenyleneiodonium (DPI) induces apoptosis by increasing mitochondrial superoxide production. Antioxidants and MnSOD mitigate this DPI-induced mitochondrial damage, suggesting a novel pathway for apoptosis signaling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A probabilistic approach to the analysis of elastic light scatter profiles for identification of culturable bacteria.

Systematic and applied microbiology·2026
Same author

CARGO: A Cytometry Analysis framework via Regularized Graph Optimal-transport.

PLoS computational biology·2026
Same author

Profiling the CFTR Variant Selectivity and Off-Target Interactions of VX-121.

bioRxiv : the preprint server for biology·2026
Same author

The impact of high fat diet on global protein abundance and fractional synthetic rate in liver and mammary gland of peak lactation ICR mice.

PloS one·2026
Same author

Gate Shape Matters.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Portable Dual-Mode Biosensor for Quantitative Determination of <i>Salmonella</i> in Lateral Flow Assays Using Machine Learning and Smartphone-Assisted Operation.

Biosensors·2026

Area of Science:

  • Biochemistry
  • Cell Biology
  • Toxicology

Background:

  • Diphenyleneiodonium (DPI) and diphenyliodonium (IDP) inhibit phagocyte NAD(P)H oxidase.
  • High concentrations of iodonium compounds can inhibit the mitochondrial respiratory chain.
  • Mitochondrial respiratory chain inhibition is linked to superoxide production and apoptosis.

Purpose of the Study:

  • Investigate the effects of iodonium compounds on mitochondria-derived superoxide production.
  • Determine the role of mitochondrial superoxide in DPI-induced apoptosis.
  • Explore potential therapeutic interventions against DPI-induced mitochondrial damage.

Main Methods:

  • Measured mitochondrial superoxide production in cultured cells and isolated rat-heart submitochondrial particles.

Related Experiment Videos

  • Assessed mitochondrial function via membrane potential monitoring.
  • Studied apoptotic pathways through cytochrome c release, caspase 3 activation, DNA fragmentation, and propidium iodide staining.

    • Main Results:

    • DPI induced mitochondrial superoxide production.
    • DPI decreased mitochondrial membrane potential and released cytochrome c, leading to apoptosis.
    • Antioxidants and MnSOD overexpression significantly reduced DPI-induced mitochondrial damage and apoptosis.

    Conclusions:

    • DPI induces apoptosis via the generation of mitochondrial superoxide.
    • Mitochondrial superoxide plays a critical role in DPI-induced apoptosis.
    • DPI-induced mitochondrial superoxide production offers a model for studying related signaling pathways.