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Interleukin-1 facilitates airway epithelial migration in response to injury
Wesley Hicks1, Leon A Hall, Debra A Tristram
1Department of Surgical Oncology, Head and Neck Surgery, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. Wesley.Hicks@roswellpark.org
Objective:
To test the hypothesis that interleukin (IL)-1 plays a permissive role in respiratory epithelial cell migration and proliferation.
Study Design:
Primary cultures of porcine respiratory epithelial cells or tracheal organ explants were cultured in the presence or absence of function-blocking antibodies to IL-1. Areas of epithelial cell outgrowth were determined in control and antibody-treated organ explants daily for 4 days. At intervals, cultured cells were collected for cell counting and viability determination. Time course and dose-response curves were constructed for control and antibody-treated groups.
Results:
Interleukin-1 secretion into culture supernatants increased sharply from days 3 to 7. Outgrowths from tracheal explants were reduced by greater than 60% by single antibody treatment, and by over 90% by treatment with antibodies to both IL-1alpha and -1beta by day 4 of culture. Function-blocking antibodies to IL-1 significantly reduced cell number by day 7 of culture.
Conclusions:
Interleukin-1 is produced by respiratory epithelial cells in culture during log phase growth and plays a permissive role in cell migration and proliferation.