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Updated: Aug 13, 2026

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
Published on: December 27, 2013
[Remote effects of local injection of botulinum toxin type A]
Objective:
To assess the severity and temporal profile of remote effects of botulinum toxin type A, BTX-A (Botox from Allergan Inc, USA and CBTX-A made by Lanzhou Biological Products Institute, China) injected locally on neuromuscular junction.
Methods:
Patients had enrolled in a prospective study for their movement disorders, 18 cases with Botox and 22 cases with CBTX-A were studied. Single fiber electromyography (SFEMG) in the extensor digitorum communis muscle or tibialis anterior muscle was performed before and 2-3 weeks, 5-8 weeks, 4-5 months after injection of Botox or CBTX-A.
Results:
It was measured totally 119 times. Significant increase of jitter was demonstrated 2-3 weeks after injections in both groups and MCD was in direct proportion to dose of injections. Fiber density value increased at the same time or later and lasted until 4-5 months after injections.
Conclusions:
Subclinical effects on neuromuscular transmission of remote uninjected muscles after injections of Botox and CBTX-A are demonstrated, which indicates that the toxin spread distantly from the site of injection. SFEMG is valuable for studying the pathogenesis of the remote effects of BTX-A and for avoiding reinjection too frequently.
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