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[6A8 cDNA encodes A type of alpha-mannosidase]
1Department of Immunology, Insitutute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Objective:
To find the nature of the protein encoded by 6A8 cDNA.
Methods:
In the light of that the ligand of Con A is mannoses and that the function of alpha-mannosidase is trimming of mannoses in the oligosaccharide chains of glycoproteins, COS-7 cells and CNE-2L2 cells, a cloned cell line of human nasopharyngeal carcinoma, were transfected with pRc/CMV-sense 6A8 cDNA or pRc/CMV-antisense 6A8 cDNA and the Con A binding to the cells was examined upon a lazer scan confocal microscope.
Results:
The data demonstrated that with transfection of pRc/CMV-sense 6A8 cDNA the binding of Con A to the cells was decreased, but the staining of the cells with mAb 6A8 was enhanced, and vise versa for the cells with transfection of pRc/CMV-antisense 6A8 cDNA.
Conclusions:
The protein encoded by 6A8 cDNA might be a type of alpha-mannosidase recognized by mAb 6A8.