Related Experiment Videos
[Study on GMA-DNA adducts in vivo]
1Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050.
Objective:
To investigate whether glycidly mechacrylate (GMA-DNA) adducts can be produced in various organs of rat in vivo.
Methods:
Rats divided into 5 groups were orally administrated with mutagen, GMA 31.25, 62.5, 125, 250 mg/kg respectively for 14 days. DNA adducts produced in liver, kidney, white blood cells and testis had been analyzed by nuclease P1 mediated 32P-postlabelling method.
Results:
Several GMA-DNA adducts were formed in various organs (in white blood cells, 4 types, liver and kidney, 3 types and testis 1 type). The amount of GMA-DNA adducts increased with GMA dosage within 0-125 mg/kg dosages, degree of the overall level of GMA-DNA adducts in various organs were kidney > liver > white blood cells > testis. N3-methacrylate-2-hdroxypropyl-deoxycytidine monophosphate was found in kidney, liver and white blood cells.
Conclusions:
GMA with electrophilic group could react with negatively charged centers on DNA and form GMA-DNA adducts.