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Updated: Sep 27, 2026

A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
[Obtaining and construction of a novel gene from Schistosoma japonicum by expressed sequence tags]
Yan Li1, Xin-bing Yu, Zhong-dao Wu
1Department of Parasitology, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080.
Objective:
To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum.
Methods:
New genes of S. japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector.
Results:
An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3.
Conclusion:
The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene's function.
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