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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
07:37

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Published on: May 22, 2018

[Comparison of circulating antigen detection in schistosomiasis japonica patients using two methods of ELISA]

L Z Qiu1, Y H Zhang, H Li

  • 1Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases
|February 6, 2003
PubMed
Abstract

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.