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[Isoelectric focusing analysis of Echinococcus multilocularis protoscolex antigens]
1Institute of Infectious and Parasitic Disease, Chongqing University of Medical Science, Chongqing 400016.
Abstract
Objective:
To understand the components of the crude antigens of Echinococcus multilocularis protoscoleces.
Methods:
Isoelectric focusing was carried out to fractionate the protein components of E. multilocularis protoscoleces. The individual fractions were collected and subjected to SDS-PAGE, the gels were analyzed by a gel analysis system.
Results:
The pI values of individual fractions varied from 4.0 to 8.5, while the molecule weights of main protein bands were 72/73, 62/63, 42/44, 35/36, 34 and 21/22 kDa.
Conclusion:
An alternative separation method of crude antigens of Echinococcus multilocularis was established.