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[Study on metabolites on aconitine in rabbit urine]
Ying Sun1, Hong-gui Zhang, Xiang-guo Shi
1Department of Natural Pharmaceutical Chemistry, School of Pharmacy, jilin University, Changchun 130021, China.
Aim:
To identify the main metabolites of aconitine in the urine of rabbits.
Methods:
After oral administration of aconitine (5 mg.kg-1), the urine of male rabbits was collected and extracted by solid phase extraction and analyzed by liquid chromatography-ion trap mass spectrometry.
Results:
Aconitine and 4 metabolites were found in the rabbit urine. Their protonated molecular ions at m/z 632, m/z 604, m/z 590, m/z 500 and multistage fragment ions with neutral loss of 60 u, 32 u, 28 u and 18 u were monitored. Their relative concentration were M1 > Aconitine > M4 > M2 > M3.
Conclusion:
The metabolites M1-M4 were deduced as 16-O-demethylaconitine, benzoylaconine, 16-O-demethylbenzoylaconine and aconine, respectively.