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Related Experiment Videos

Bone loss detection in rats using a mouse densitometer.

N Binkley1, D B Dahl, J Engelke

  • 1University of Wisconsin Institute on Aging, University of Wisconsin, Madison, Wisconsin 53706, USA. nbinkley@facstaff.wisc.edu

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|February 6, 2003
PubMed
Summary

The portable PIXImus densitometer effectively detects ovariectomized (ovx)-induced bone loss in rats, comparable to standard DXA methods. Its speed and portability make it valuable for studying rat osteopenia.

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Area of Science:

  • Osteoporosis research
  • Animal models
  • Bone densitometry

Background:

  • Ovariectomized (ovx) rats are standard models for estrogen-depletion bone loss.
  • Dual-energy X-ray absorptiometry (DXA) is commonly used to measure bone mineral density.
  • The utility of the portable PIXImus densitometer in rat studies is not well-established.

Purpose of the Study:

  • To compare the PIXImus and DPXL densitometers in detecting ovx-induced bone loss in rats.
  • To assess the accuracy of the PIXImus by correlating measurements with ash weight.
  • To evaluate the effectiveness of different distal femur regions of interest (ROI) for detecting bone loss.

Main Methods:

  • Twenty-three female Sprague-Dawley rats were assigned to sham or ovx groups.

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  • Bone mineral density was measured in vivo and ex vivo using PIXImus and DPXL densitometers at multiple time points.
  • Instrument accuracy was validated against ash weight measurements.

    • Main Results:

    • Both PIXImus and DPXL detected significant bone loss by 1 month post-ovx.
    • A 4-mm ROI on the PIXImus showed greater bone loss detection than an 8-mm ROI.
    • PIXImus and DPXL measurements of bone loss at 2 months were highly comparable (22.2% vs. 22.4%).
    • PIXImus overestimates bone mineral content but shows excellent correlation with DPXL and ash weight.

    Conclusions:

    • The PIXImus densitometer is a viable tool for assessing ovx-induced osteopenia in rats.
    • Its portability and speed offer advantages for bone loss studies in this animal model.
    • The PIXImus provides results comparable to standard DXA, supporting its use in research.