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Effect of C-peptide on wound healing and microcirculation in diabetic mice
S Langer1, F Born, A Breidenbach
1Clinic for Plastic and Hand Surgery, Burn Center, Ruhr University Bochum, Germany. Stefan.Langer@ruhr-uni-bochum.de
Aim:
Recent studies have demonstrated that C-peptide is biologically active and might have a beneficial effect on late complications in diabetes mellitus. The aim of this study was to investigate the effects of systemically given C-peptide on dermal wound healing in diabetic mice.
Methods:
Experiments were carried out in male SKH-1 hr hairless mice. Dermal wounds were created (diameter 2.5mm) in streptozotozin-diabetic and normal control mice. Mice were randomized into three treatment groups (n = 10 each): Normal controls, diabetic mice with PBS or C-peptide injection twice daily. At various time points (prior wounding as well as days 4, 7, 10 and 15) microcirculation was quantitatively analyzed by intravital fluorescent microscopy to determine wound surface area, vessel diameter, red blood cell velocity, plasma leakage, functional capillary density. In addition, leukocyte/endothelium interaction was quantified by in vivo visualization of leukocytes.
Results:
Systemic administration of C-peptide showed no influence on wound healing or standard microcirculatory parameters. The leukocyte/ endothelium interaction revealed a significant (p<0.05) increase in the number of adherent leukocytes 15 days after wound creation in C-peptide treated diabetic mice.
Conclusion:
Except for the significantly increased number of leukocytes adherent to venular endothelium in the C-peptide group no alteration was observed in wound healing and microcirculation. Neutrophil recruitment after C-peptide injection is of interest because it may reduce the risk of infection in diabetes mellitus.
Insights
C-peptide did not improve wound healing in diabetic mice. However, it increased leukocyte adhesion, which may help reduce infection risk in diabetes mellitus.
Area of Science:
- Diabetes Mellitus Research
- Wound Healing Studies
- Endocrinology
Background:
- C-peptide is biologically active and may benefit diabetes complications.
- Investigating C-peptide's role in dermal wound healing in diabetic models is crucial.
Purpose of the Study:
- To evaluate the systemic effects of C-peptide on dermal wound healing in diabetic mice.
- To analyze microcirculatory parameters and leukocyte-endothelium interactions.
Main Methods:
- Dermal wounds were created in streptozotocin-diabetic and control mice.
- Mice received C-peptide or PBS injections.
- Microcirculation and leukocyte-endothelium interactions were assessed using intravital fluorescent microscopy.
Main Results:
- C-peptide administration did not affect wound healing or standard microcirculatory parameters.
- A significant increase in adherent leukocytes was observed in C-peptide treated diabetic mice 15 days post-wounding.
Conclusions:
- C-peptide did not alter wound healing or microcirculation in diabetic mice.
- Increased neutrophil recruitment suggests a potential benefit in reducing infection risk in diabetes.