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Related Experiment Videos

Background and operating parameters in microemulsion electrokinetic chromatography.

Kevin D Altria1, Pierre-Etienne Mahuzier, Brian J Clark

  • 1GlaxoSmithKline Research and Development, Ware, Herts, UK. kevin.d.altria@gsk.com

Electrophoresis
|February 6, 2003
PubMed
Summary

Microemulsion electrokinetic chromatography (MEEKC) uses oil droplets in aqueous buffers for separations. This review covers MEEKC methods, compositions, and operating variables for effective analysis.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique.
  • Separations utilize microemulsions: surfactant-coated nanometer-sized oil droplets in aqueous buffer.
  • Cosurfactants, like short-chain alcohols, stabilize these microemulsions.

Purpose of the Study:

  • To review microemulsion types and compositions used in MEEKC.
  • To describe the effects of key operating variables on MEEKC performance.
  • To cover specific applications, including water-in-oil microemulsions and chiral separations.

Main Methods:

  • Review of existing literature on MEEKC techniques.
  • Analysis of microemulsion formulations (surfactant, cosurfactant, oil, buffer).

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  • Examination of operating parameters: pH, temperature, solvent additions, cyclodextrins.

    • Main Results:

    • Various microemulsion compositions and types are suitable for MEEKC.
    • Operating variables significantly influence separation efficiency and selectivity.
    • MEEKC can achieve effective separations, including chiral resolutions.

    Conclusions:

    • MEEKC is a versatile electrodriven separation technique.
    • Optimization of microemulsion composition and operating conditions is crucial for MEEKC.
    • MEEKC demonstrates potential for complex mixture analysis and chiral separations.