[Distribution of human hsp90 beta gene in active chromatin upon heat shock]

To study the distribution of human hsp90 beta gene in active chromatin of Jurkat cells subjected to heat shock.

Active and inactive chromatin were isolated by using organomercurial affinity chromatography from Jurkat cells with or without heat shock. Slot-blot hybridization was then carried out with human hsp90 beta cDNA probe. And the efficiency of mRNA expression was studied by Northern blot hybridization.

The content of the hsp90 beta gene distributed in the active chromatin was increased upon heat shock which was comparable with the highly efficient mRNA expression induced by the same treatment.

The results suggested chromatin activity was a prerequisite for heat shock induction of hsp90 beta gene.