[Effect of 15-methyl PGF2 alpha methyl ester combined with tyrosine hydrazide on early pregnancy in mice]
Related Experiment Video
Updated: Sep 27, 2026
Is My Mouse Pregnant? High-Frequency Ultrasound Assessment
Published on: March 18, 2021
1Department 2 of Pharmacology, Institute of Materia Medica, CAMS and PUMC, Beijing 100050.
Objective:
To observe the effect of 15-methyl PGF2 alpha methyl ester (PG05) in combination with tyrosine hydrazide (TH) on early pregnancy in mice.
Methods:
The pregnancy rate of mice treated with PG05 and TH was counted and serum progesterone level was measured by RIA.
Results:
Administration of TH at 90 mg.kg.day-1 to mice on day 7-9 of gestation enhanced significantly the termination of early pregnancy induced by PG05 at 0.2 mg-1.kg-1 for 2 days. Serum progesterone level of mice treated with PG05 combined with TH was found to be markedly lower than that of animals given PG05 or TH alone. TH at 30 micrograms-1.ml-1 inhibited significantly the activity of 3 beta-HSD of luteal cells in rat. However, PG05 at 10 micrograms-1.ml-1 had no influence upon inhibitory effect of 3 beta-HSD activity induced by TH. TH at 150 mg-1.kg-1 for 3 days did not affect the progesterone receptor level of utering cytoplasma in pseudopregnant rats.
Conclusions:
The inhibitory effect of TH on the production of progesterone may be the main mechanism to terminate early pregnancy when used synergically with PG05.
More Related Videos
09:04Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods
Published on: September 10, 2018
08:53Generation of the Early-Gestational Maternal Immune Activation Mouse Model to Assess Prenatal Inflammation on Neurodevelopment
Published on: March 24, 2026