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[Tolerance of HepG2 and HepG2.2.15 cell lines to different apoptotic stimuli]
1Department of Infectious Diseases of Nanfang Hospital, First Military Medical University, Guangzhou 510515.
Objective:
To elucidate the effect of HBV on hepatocyte apoptosis.
Methods:
The HepG2 cells and the HBV transfected HepG2.2.15 cells were cultured with MIX or ActD or cultured in DMEM medium without serum, The apoptosis was examined with FCM.
Results:
The apoptotic rates of HepG2.2.15 cells at 24 h and 48 h after MTX addition were 10.8% and 13.3%, at 24 h and 48 h after Act D addition were 16.8% and 37.7%, and at 4th and 6th day after serum withdrawal were 13.2% and 14.8%, respectively. While those of HepG2 cells were 12.6% and 65.3%, 44.5% and 89.7%, and 19.8% and 28.8%, correspondingly.
Conclusion:
HepG2.2.15 cell was tolerant to these apoptotic stimuli, and it might be inferred that HBV inhibits hepatocyte apoptosis.