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[Identification and characterization of HIV-1 subtype D strain in China]
1AIDS Reference Laboratory, National Center for AIDS Prevention and Control, Chinese Academy of Preventive Medicine, Beijing 100050.
Objective:
Through sequence analysis of HIV-1 gag, tat and env regions, the subtype D HIV-1 had been identified appering in China.
Methods:
The blood was collected from HIV-1 infected individual (SC9 712) in Sichuan Province. The peripheral blod mononuclear cells (PBMCs) were isolated from the blood by ficoll and the genomic DNA was extracted. The gag gene, the C2-V3 of the env gene and the first exon of tat gene were amplified by nested-PCR and sequenced directly.
Results:
The genetic distance respectively is 7.56% in tat gene between SC9 712 and UG274A, 9.59% and 9.94% in gag gene between SC9 712 and K31 and VI203, 13.86% in env gene between SC9 712 and MAL. On the phylogenetic tree the SC9 712 HIV-1 strain clustered with the Africa subtype D strains UG274A, K31, VI203 and MAL. The above data demonstrated that SC9 712 was subtype D HIV-1 strain. Epidemiological data showed that SC9 712 virus was from a person who had worked in Africa.
Conclusion:
The results indicated that the person who came back from Africa had transmitted the subtype D of HIV-1 from Africa to China.