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[Comparison of three methods in detection of rotavirus infection in neonates]
Insights
Enzyme-linked immunosorbent assay (ELISA) showed a high false positive rate for rotavirus infection in neonates. Polyacrylamide gel electrophoresis (PAGE) is a more reliable method for diagnosing rotavirus in infants.
Area of Science:
- Virology
- Pediatric Gastroenterology
- Diagnostic Microbiology
Background:
- Rotavirus is a leading cause of acute gastroenteritis in infants.
- Accurate and reliable detection methods are crucial for diagnosing rotavirus infection in neonates.
- Comparing diagnostic techniques is essential for clinical practice.
Purpose of the Study:
- To compare the diagnostic accuracy of three methods for rotavirus detection in neonates.
- To evaluate Enzyme-linked immunosorbent assay (ELISA), Polyacrylamide gel electrophoresis (PAGE), and Electron Microscopy (EM).
Main Methods:
- Fecal samples from 252 neonates and infants (<4 months) were analyzed.
- Samples were tested using ELISA, PAGE, and EM.
- Comparison of results based on age groups and clinical presentation.
Main Results:
- ELISA showed a high positive rate (54.6%) in healthy neonates, suggesting false positives.
- PAGE results were often negative when ELISA was positive in young infants.
- Coincident detection rates between ELISA and PAGE increased with infant age.
- PAGE demonstrated higher reliability and objectivity compared to ELISA.
Conclusions:
- ELISA exhibits a significant false positive rate in rotavirus detection among neonates.
- PAGE is a more reliable and objective method for diagnosing rotavirus in neonates.
- Diagnostic method choice may depend on infant age and clinical context.
Objective:
To compare, three methods in detection of rotavirus infection in neonates.
Methods:
252 fecal samples from neonates with acute gastroenteritis, healthy neonates and under 4-month-old babies with diarrhoea were detected with ELISA, PAGE and EM.
Results:
Using ELISA and PAGE to check 5 fecal samples from neonates with diarrhoea, the positive number of ELISA was 4 and the PAGE was all negative. One fecal sample from neonate of one month old with diarrhoea detected with ELISA was strong positive and result with EM was negative. By checking 119 fecal samples from normal neonates of less than ten days old, the positive rate of ELISA was 54.6% and results of PAGE were all RNA negative. 128 fecal samples from less than 4-month-old babies with diarrhoea showed coincident detection rate of ELISA and PAGE (81.2%), but which was going up with increasing of their ages. We also checked 55 fecal samples from less than 4-month-old babies with diarrhoea by ELISA, PAGE, EM and the positive rates were 27.3%, 38.2% and 20% respectively, the coincident rate of the three was 63.6% and the differentiation rate 36.4%.
Conclusion:
The differentiation rate between ELISA and PAGE was obvious, as in the elisa there showed a sirong positive and in PAGE the weak. The diffesence went down with increasing of the ages of the babies with diarrhoea. This indicated that the false positive rate of ELISA existed. PAGE is more reliable and objective and is the better method to check neonate diarrhoea.